CRISPR-dPCR quantifies CRISPR knock-in by distinguishing integration from donor abundance and background, using multiplex digital PCR with orthogonal strategies.

bioRxiv · Oct 27, 2026 · PreprintMolecular Biology

A lipid nanoparticle platform for high yield CRISPR-targeted homology directed repair enables fully non-viral CAR T cell generation

Joy J. Chen, Atip Lawanprasert, A.L.W. Kleinhenz, Mehar G. D. Namala, Daniel Chu, York Tang, Valentine Lindarto, Michael Launspach, Danna Lee, Hao Wu, Niren Murthy, David N. Nguyen

This paper develops a lipid nanoparticle (LNP) platform for non-viral CRISPR-mediated homology directed repair (HDR) to generate CAR T cells with improved efficiency and reduced toxicity compared to electroporation.

bioRxiv · Aug 5, 2026 · PreprintBioengineering

Loss of Mast cells and histaminergic signaling link diet to platelet-mediated NETosis and mammary cancer recurrence

Claire P. Schane, Adam T. Nelczyk, C Chen, Jiyoung Seo, Yu Wang, Natalia Krawczyńska, Shruti V. Bendre, Erin Weisser, Dhanya Pradeep, Hashni Epa Vidana Gamage, Lara I. Kockaya, Y. Fei, Anasuya Das Gupta, Hannah Kim, Madeline A. Henn, , Erik Russell Nelson

This study links diet to mammary cancer recurrence via mast cell loss and histaminergic signaling, showing that H2R antagonists promote platelet-mediated NETosis and reemergence from dormancy.

bioRxiv · Apr 15, 2026 · PreprintCancer Biology