Engineered transcription-associated Cas9 targeting in eukaryotic cells
Gregory W. Goldberg, Manjunatha Kogenaru, Sarah Keegan, Max A. B. Haase, Larisa Kagermazova, Mauricio A. Arias, Kenenna Onyebeke, Samantha Adams, Daniel K. Beyer, David Fenyö, Marcus B. Noyes, Jef D. Boeke
This paper introduces a method to temporally control Cas9 DNA targeting by coupling it with transcription through engineered RNA binding, enabling selective gene editing in yeast and human cells.
Nature Communications · Nov 27, 2024Peer-reviewedMolecular Biology