Determination of the cellular target engagement by direct-to-biology Cellular Thermal Shift Assay (CETSA)
Magdalena M. Szewczyk, Darshil Patel, Sarvatit Patel, Aiymzhan Istayeva, Robert A. Batey, Dalia Barsyte-Lovejoy, Vijayaratnam Santhakumar
This paper demonstrates a direct-to-biology CETSA approach to evaluate cellular target engagement without purification, showing unpurified compounds can be used for assessment.
bioRxiv · Mar 2, 2026 · PreprintCell Biology