Magdalena M. Szewczyk

Chemically induced USP21-KLHL12 association recruits USP21 to COPII assembly

The paper shows that BAY-805 induces USP21-KLHL12 association, recruiting USP21 to COPII vesicles and impairing large cargo transport.

ABHD2 activity is not required for the non-genomic action of progesterone on human sperm

This study shows that ABHD2 activity is not required for progesterone-induced Ca2+ influx via CatSper and subsequent motility changes in human sperm.

Direct-to-Biology Enables Rapid Identification of Potent FBXO22 Degraders

The paper tests PROTACs targeting FBXO22 and finds no degradation of four targets but identifies potent FBXO22 homo-PROTACs and CRBN/VHL-mediated degraders.

Determination of the cellular target engagement by direct-to-biology Cellular Thermal Shift Assay (CETSA)

This paper demonstrates a direct-to-biology CETSA approach to evaluate cellular target engagement without purification, showing unpurified compounds can be used for assessment.

Development of a dual chemical probe for the USP16 and HDAC6 zinc-finger ubiquitin-binding domain

The paper develops a chemical probe targeting the USP16 zinc-finger ubiquitin-binding domain and evaluates its selectivity and activity in cellular assays.

Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells

The paper identifies UNC10013, a covalent ligand targeting SETDB1's 3TD domain, which reduces methyltransferase activity and shows selectivity in cells.

A chemical probe to modulate human GID4 Pro/N-degron interactions

The paper introduces PFI-7, a chemical probe that inhibits GID4 Pro/N-degron interactions, revealing non-degradative roles of the CTLH complex in human cells.