Takeshi Shimi

Nuclear wrinkles result from geometric adaptations to cellular and nuclear morphological changes in epithelial cells

The paper investigates nuclear wrinkles in epithelial cells, finding they form due to geometric adaptations to mechanical stress and cell shape changes, without causing damage.

A Nanoheater-Integrated Fluorescence Lifetime Thermometer for Investigating Subcellular Heat Shock Factor 1 Responses

This paper develops a nanoheater-thermometer to measure subcellular temperature and monitor HSF1 responses in living cells, showing that localized heating induces HSF1 foci at lower temperatures than whole-cell heating.

OptoChaperone - A biohybrid tool for regulating protein condensates in cells and in vitro

OptoChaperone enables reversible, spatiotemporal control of protein condensates using light, demonstrating efficacy in regulating disease-related condensates without chemical additives or genetic modifications.

Probing nanomechanics by direct indentation using Nanoendoscopy-AFM reveals the nuclear elasticity transition in cancer cells

This study uses Nanoendoscopy-AFM to directly measure nuclear elasticity in cancer cells, finding increased elasticity under serum depletion and decreased elasticity with TGF-β treatment.

Adaptive-Resolution Multi-Orientation Analysis of Complex Filamentous Network Images

The paper introduces an image analysis method to segment filamentous structures and their intersections in microscopy images by resolving multiple orientations.