Dynamic network-guided CRISPRi screen reveals CTCF loop-constrained nonlinear enhancer-gene regulatory activity in cell state transitions
Renhe Luo, Jielin Yan, Jin Woo Oh, Xi Wang, Dustin Shigaki, Wilfred Wong, Hyunwoo Cho, Dylan Murphy, Ronald R. Cutler, Bess P. Rosen, Julián Pulecio, Dapeng Yang, Rachel A. Glenn, Tingxu Chen, Qing V. Li, , M Beer
The paper uses a CRISPRi screen in hESC differentiation to identify enhancers with nonlinear regulatory activity, revealing a CTCF loop-constrained model for improved enhancer prediction.
Nature Genetics · Jul 24, 2023Peer-reviewedGenetics