Hyunwoo Cho

The paper identifies pre-established chromatin regions in human ESCs that enable rapid activation of lineage-specific genes during differentiation.

This paper develops a human organoid model of early PDAC that identifies genetic and epigenetic barriers to lineage plasticity, showing CDKN2A loss is essential with KRAS/TP53 mutations and TET1 suppression links ERK signaling to transcription factor silencing.

This study uses Perturb-seq to analyze how varying levels of pluripotency regulators affect hESC self-renewal and differentiation through single-cell RNA-seq.

This study improves the yield and subtype identity of hPSC-derived midbrain dopamine neurons through optimized WNT and FGF18 signaling modulation, enhancing their functional and transcriptional similarity to primary neurons.

The paper uses CRISPR-Cas9 screens to identify genes affecting stem cell fitness versus pluripotency identity, finding distinct and overlapping roles for chromatin and metabolic regulators.

The paper identifies zinc finger proteins that establish distinct chromatin boundaries at Hox clusters during development, working with cohesin to regulate cell fate.

The paper identifies a long-range enhancer for ONECUT1 involved in pancreatic differentiation and links a diabetes risk variant to disrupted transcription factor binding.

The paper identifies p53-mediated apoptosis and TNF-NFkB signaling as key factors limiting survival of grafted dopamine neurons and shows that adalimumab treatment improves their engraftment and function in a PD mouse model.

This paper establishes a pluripotent stem cell platform using genetically diverse mouse lines to study the impact of natural genetic variation on development and gene regulation.

Chromatin regulators including EZH2, EHMT1/2, and DOT1L establish an epigenetic barrier in human neural progenitors that poises maturation genes and sets the protracted pace of cortical neuron maturation.

The paper uses a CRISPRi screen in hESC differentiation to identify enhancers with nonlinear regulatory activity, revealing a CTCF loop-constrained model for improved enhancer prediction.

The paper presents optimized protocols for generating definitive endoderm and forebrain-patterned neural organoids from mouse epiblast stem cells.