E. K. Mahlandt

The paper introduces a method to recruit proteins to GFP-tagged targets using an antiGFP nanobody fused to the iLID system, enabling light-dependent localization without modifying the iLID.

This paper develops Opto-RhoGEFs, an optogenetic tool to control Rho GTPase activity in endothelial cells, revealing a VE-cadherin-independent mechanism for regulating vascular barrier strength.

The paper characterizes and optimizes relocation-based biosensors for Rac and Cdc42, improving their specificity and efficiency for cell-based applications.

This study identifies ARHGAP17 as a regulator of Cdc42 activity at invadopodia, showing it controls spatiotemporal Cdc42 activity during invadopodia formation and disassembly.

This paper introduces a new calcium biosensor based on fluorescence lifetime imaging, which provides accurate and pH-insensitive quantification of intracellular calcium concentrations.

The paper provides methods to process and analyze timelapse imaging data for quantitative understanding of biological dynamics.

The paper improves RhoA localization-based biosensors to better detect endogenous RhoA activity in various cellular processes.

The paper identifies endothelial junctional membrane protrusions as preferred sites for neutrophil transmigration during inflammation and shows they depend on Rac1 activity.