J. Goedhart

Biosensor imaging of G protein mediated Signaling Pathways Activated Immediately Downstream of CaSR

The paper uses biosensors to study CaSR-mediated signaling pathways in HEK-293 cells, showing activation of Gq and Gi proteins and their effects on intracellular calcium and cAMP levels.

Studentsourcing - aggregating and re-using data from a practical cell biology course

The paper introduces studentsourcing, a method to collect, aggregate, and reuse data from a cell biology course, demonstrating its utility for both teaching and scientific research.

Trade-offs between cost and information in cellular prediction

The paper shows that cellular networks can approach predictive information bounds for specific signal types but face trade-offs between information gain and cost, with implications for bacterial chemotaxis.

iLID-antiGFP-nanobody is a flexible targeting strategy for recruitment to GFP-tagged proteins

The paper introduces a method to recruit proteins to GFP-tagged targets using an antiGFP nanobody fused to the iLID system, enabling light-dependent localization without modifying the iLID.

Opto-RhoGEFs: an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

This paper develops Opto-RhoGEFs, an optogenetic tool to control Rho GTPase activity in endothelial cells, revealing a VE-cadherin-independent mechanism for regulating vascular barrier strength.

Cell-based optimisation and characterisation of genetically encoded, location-based biosensors for Cdc42 or Rac activity

The paper characterizes and optimizes relocation-based biosensors for Rac and Cdc42, improving their specificity and efficiency for cell-based applications.

ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia

This study identifies ARHGAP17 as a regulator of Cdc42 activity at invadopodia, showing it controls spatiotemporal Cdc42 activity during invadopodia formation and disassembly.

Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1

The paper identifies endothelial transmigration hotspots that limit vascular leakage by heterogeneous ICAM-1 expression, showing that ICAM-1 distribution determines hotspot location and function.

Heterogeneity and dynamics of ERK and Akt activation by G protein-coupled receptors depend on the activated heterotrimeric G proteins

The paper investigates cell-to-cell heterogeneity in ERK and Akt activation by GPCRs, showing distinct dynamics depending on the activated G proteins.

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

This paper introduces a new calcium biosensor based on fluorescence lifetime imaging, which provides accurate and pH-insensitive quantification of intracellular calcium concentrations.

Visualizing and quantifying data from timelapse imaging experiments

The paper provides methods to process and analyze timelapse imaging data for quantitative understanding of biological dynamics.

Visualizing endogenous RhoA activity with an improved localization-based, genetically encoded biosensor

The paper improves RhoA localization-based biosensors to better detect endogenous RhoA activity in various cellular processes.

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration

The paper identifies endothelial junctional membrane protrusions as preferred sites for neutrophil transmigration during inflammation and shows they depend on Rac1 activity.

SuperPlotsOfData - a web app for the transparent display and quantitative comparison of continuous data from discrete conditions

The paper introduces a web app for transparently displaying and comparing continuous data from discrete conditions using advanced visualization techniques.

A new FRET biosensor enlightens cAMP signalling in budding yeast

The paper develops a FRET biosensor, yEPAC, to measure cAMP signaling in budding yeast and finds that cAMP peaks vary with nutrient transitions and follow Weber's law.

Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells

PlotTwist - a web app for plotting and annotating time-series data

PlotTwist is a web app that simplifies time-series data visualization and annotation without coding, offering normalization, multiple display formats, and annotation tools.

PlotsOfDifferences - a web app for the quantitative comparison of unpaired data

PlotsOfDifferences is a web tool that calculates effect sizes and their compatibility intervals for unpaired data using bootstrapping, providing graphical and tabular outputs with optional p-values.

Selectivity of Guanine Nucleotide Exchange Factor-mediated Cdc42 activation in primary human endothelial cells

The paper quantifies Cdc42 activation by endothelial GEFs in primary human cells, identifying FGD1 as the most selective activator.

PlotsOfData - a web app for visualizing data together with its summaries

PlotsOfData is a web app that visualizes raw data with statistical summaries using ggplot2, offering flexibility in data display and interpretation.

Superfolder mTurquoise2ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets.

The paper develops a new fluorescent protein variant, sfTq2ox, optimized for the bacterial periplasm to enable efficient FRET for studying cell division antibiotic targets.

A FRET based biosensor for measuring Gα13 activation in single cells

The paper develops a FRET-based biosensor to measure Gα13 activation in single cells and demonstrates its use in detecting receptor-induced responses.

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

The paper evaluates fluorescent proteins as FRET acceptors for mTurquoise2, identifying mNeonGreen as the most efficient and photostable pair.

Palmitoylation of the oncogenic RhoGEF TGAT is dispensable for membrane localization and consequent activation of RhoA

This study shows that membrane association of TGAT is critical for its activity, but palmitoylation is not required for membrane localization or RhoA activation.