Opto-RhoGEFs: an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength
E. K. Mahlandt, S. Palacios Martinez, J. J. G. Arts, S. Tol, J. D. van Buul, J. Goedhart
This paper develops Opto-RhoGEFs, an optogenetic tool to control Rho GTPase activity in endothelial cells, revealing a VE-cadherin-independent mechanism for regulating vascular barrier strength.
bioRxiv · Oct 17, 2022Cell Biology