Laboratory evolution and polyploid SCRaMbLE reveal genomic plasticity to synthetic chromosome defects and rearrangements
Thomas C. Williams, Heinrich Kroukamp, Xin Xu, Elizabeth L. I. Wightman, Briardo Llorente, Anthony R. Borneman, Alexander C. Carpenter, Niël van Wyk, Monica I. Espinosa, Elizabeth L. Daniel, Roy S. K. Walker, Yizhi Cai, Helena K. M. Nevalainen, Natalie C. Curach, Ira W. Deveson, , Ian T. Paulsen
The paper debugs a synthetic yeast chromosome and uses laboratory evolution to identify genomic rearrangements that suppress growth defects caused by synthetic modifications.
bioRxiv · Jul 22, 2022 · PreprintSynthetic Biology