The apparent loss of PRC2 chromatin occupancy as an artefact of RNA depletion
Evan Healy, Qi Zhang, Emma H. Gail, Samuel C. Agius, Guizhi Sun, Michael J. Bullen, Varun Pandey, Partha Pratim Das, Jose Maria Polo, Chen Davidovich
The paper shows that RNase A treatment during ChIP-seq falsely reduces PRC2 chromatin occupancy signals, revealing limitations in using this method to study RNA-dependent chromatin regulation.
Cell Reports · Feb 27, 2024Peer-reviewedMolecular Biology