Guizhi Sun

Simultaneous single-cell profiling of the transcriptome and proteome

This paper describes a method to simultaneously profile the transcriptome and proteome from single cells, showing moderate correlation between the two modalities.

Observing concurrent subcellular dynamics in large living tissues

This paper introduces Airy beam light sheet microscopy to image subcellular dynamics in large tissues with high spatial and temporal resolution across multiple organisms and biological processes.

Contemporary seasonal human coronaviruses display differences in cellular tropism compared to laboratory-adapted reference strains

This study isolates and characterizes contemporary seasonal human coronaviruses, showing differences in cell tropism and growth compared to lab-adapted strains.

Hippo signalling regulates the nuclear behaviour and DNA dwell times of YAP and TEAD to control transcription

This study uses live microscopy to show that Hippo signalling regulates transcription by controlling the DNA dwell times of YAP and TEAD proteins.

The apparent loss of PRC2 chromatin occupancy as an artefact of RNA depletion

The paper shows that RNase A treatment during ChIP-seq falsely reduces PRC2 chromatin occupancy signals, revealing limitations in using this method to study RNA-dependent chromatin regulation.

Integration of Xeno-Free Single-cell Cloning in CRISPR-mediated DNA Editing of Human iPSCs Improves Homogeneity and Methodological Efficiency of Cellular Disease Modelling

This paper introduces a xeno-free single-cell cloning method for CRISPR-edited human iPSCs, achieving high clonal survival and editing efficiency for improved disease modeling.

Mouse and human microglial phenotypes in Alzheimer’s disease are controlled by amyloid plaque phagocytosis through Hif1α

This study identifies that amyloid plaque phagocytosis through Hif1α controls microglial phenotypes in Alzheimer’s disease in both mice and humans.

A single cell brain atlas in human Alzheimer’s disease

This paper creates a single-cell brain atlas of Alzheimer’s disease, identifying cell-type specific gene expression patterns and regulatory networks associated with disease.