Trioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks
Clinton Yu, Eric J. Novitsky, Sree Ganesh Balasubramani, Xiaorong Wang, Xiyu Shen, Qin Yang, Scott D. Rychnovsky, Ignacia Echeverria, Lan Huang
This paper introduces a novel trioxane-based cross-linker, TSTO, for XL-MS that enables fast and unambiguous identification of multimeric protein interactions, revealing new structural details of protein complexes.
Nature Communications · Jul 1, 2025Peer-reviewedBiochemistry