Lan Huang

Optimization of Search and Acquisition Strategies for DSSO Crosslinking Mass Spectrometry using Protein Prospector and Touchstone.

The paper evaluates DSSO crosslinking strategies using Protein Prospector and Touchstone, finding that DSSO's advantage comes from backbone fragmentation, not diagnostic ions, and that MS3 is better for proteome-scale samples.

Orthosteric Molecular Glue Inhibits COP9 Signalosome with Substrate-Dependent Potency

This paper shows that an orthosteric inhibitor of the COP9 signalosome achieves high potency through a molecular glue mechanism that enhances substrate-dependent binding.

Trioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks

This paper introduces a novel trioxane-based cross-linker, TSTO, for XL-MS that enables fast and unambiguous identification of multimeric protein interactions, revealing new structural details of protein complexes.

NEMF-mediated CAT-tailing defines distinct branches of translocation-associated quality control

The paper identifies NEMF-mediated CAT-tailing as a mechanism that directs substrates through distinct degradation pathways during translocation-associated quality control.

Molecular mechanisms for activation of the 26S proteasome

The paper investigates how ZFAND5 activates the 26S proteasome by inducing conformational changes that enhance substrate degradation.

Discovery, Structure, and Function of Filamentous 3-Methylcrotonyl-CoA Carboxylase

This study discovers that native MCC from Leishmania tarentolae forms filaments, determines its structure, and proposes a new model for its catalytic mechanism.

In vivo assembly and trafficking of olfactory Ionotropic Receptors

This study investigates the role of the co-receptor's ligand-binding domain in the assembly and trafficking of olfactory Ionotropic Receptors, revealing its importance in endoplasmic reticulum exit.