Clinton Yu

Optimization of Search and Acquisition Strategies for DSSO Crosslinking Mass Spectrometry using Protein Prospector and Touchstone.

The paper evaluates DSSO crosslinking strategies using Protein Prospector and Touchstone, finding that DSSO's advantage comes from backbone fragmentation, not diagnostic ions, and that MS3 is better for proteome-scale samples.

A meiotic B-type cyclin selectively drives mitotic progression and fidelity in cancer cells

This study shows cyclin B3-CDK1/2 drives mitotic progression and fidelity in cancer cells, with overexpression accelerating mitosis and depletion disrupting it in multiple cancer models.

Extending structural surfaceomics to identify aberrant conformations of tumor surface proteins as potential immunotherapy targets

This paper expands structural surfaceomics to identify cancer-specific protein conformations across multiple tumor models, revealing potential immunotherapy targets through crosslinking mass spectrometry and structural modeling.

LENG8 mediates RNA nuclear retention and degradation in eukaryotes

This study identifies LENG8 as a conserved RNA nuclear retention factor that prevents misprocessed mRNAs and noncoding RNAs from being exported, ensuring proper nuclear quality control.

Orthosteric Molecular Glue Inhibits COP9 Signalosome with Substrate-Dependent Potency

This paper shows that an orthosteric inhibitor of the COP9 signalosome achieves high potency through a molecular glue mechanism that enhances substrate-dependent binding.

Trioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks

This paper introduces a novel trioxane-based cross-linker, TSTO, for XL-MS that enables fast and unambiguous identification of multimeric protein interactions, revealing new structural details of protein complexes.

NEMF-mediated CAT-tailing defines distinct branches of translocation-associated quality control

The paper identifies NEMF-mediated CAT-tailing as a mechanism that directs substrates through distinct degradation pathways during translocation-associated quality control.

Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein Neddylation

This study identifies PHD2 interaction hotspots and reveals its role in regulating protein neddylation through interaction with the COP9 Signalosome complex.

Discovery, Structure, and Function of Filamentous 3-Methylcrotonyl-CoA Carboxylase

This study discovers that native MCC from Leishmania tarentolae forms filaments, determines its structure, and proposes a new model for its catalytic mechanism.

Pentatricopeptide repeat poly(A) binding protein from mitochondria of trypanosomes

The paper identifies KPAF4, a mitochondrial PABP in Trypanosoma brucei, which regulates mRNA editing and 3' modification by preventing premature A/U-tailing and ensuring transcript stability.