Cell-free pathway prototyping enables cost-effective biomanufacturing of 1,2,4-butanetriol at the 1-L scale
Blake J. Rasor, Katherine A. Rhea, Isaiah D Richardson, John Tyler Lazar, Marilyn F. Slininger Lee, Ethan N. Walters, John R. Biondo, F. Joseph Kragl, David Garcia, Kyle Zolkin, John P. Davies, Matthew W. Lux, Ashty S. Karim, Michael C. Jewett
This paper develops a cell-free biosynthesis system for 1,2,4-butanetriol, achieving high productivity and yield at scale while reducing costs.
bioRxiv · Aug 4, 2026 · PreprintSynthetic Biology