Marilyn F. Slininger Lee

Cell-free pathway prototyping enables cost-effective biomanufacturing of 1,2,4-butanetriol at the 1-L scale

This paper develops a cell-free biosynthesis system for 1,2,4-butanetriol, achieving high productivity and yield at scale while reducing costs.

Self-assembling protein materials with genetically programmable morphology and size

This study investigates how sequence variations in BMC-H proteins influence their self-assembly into different supramolecular structures using cell-free synthesis and microscopy.

Semi-automated Production of Cell-free Biosensors

This paper develops a semi-automated method for manufacturing cell-free biosensors using liquid handling robotics, demonstrating comparable performance to manual methods.

Cell-Free Protein Expression in Polymer Materials

This paper demonstrates that dried cell-free protein synthesis reactions can withstand harsh conditions during polymer casting, enabling the creation of functional materials with spatially patterned genetic capabilities.

Engineered shell proteins confer improved encapsulated pathway behavior in a bacterial microcompartment

The paper shows that modifying shell proteins of bacterial microcompartments can improve metabolite diffusion and enhance pathway function in synthetic organelles.