Author

John R. Biondo

United States Army Combat Capabilities Development Command

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Synthetic Biology· 3 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:benchmarking· 1 Research Focus:host-pathogen & immune response· 1 Assay:proteomics· 1 Approach:statistical model· 1

Frequent Co-Authors

Katherine A. RheaiD· 3 shared Marilyn F. Slininger LeeiD· 3 shared Matthew W. LuxiD· 3 shared Aleksandr E. MiklosiD· 2 shared Ashty S. KarimiD· 2 shared Casey B. BernhardsiD· 2 shared Daniel A. PhillipsiD· 2 shared Jennifer A. LeeiD· 2 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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3 shared papersSynthetic Biology

Katherine A. RheaORCID

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Author profile →

4 papers

Recent·Most cited

Cell-free pathway prototyping enables cost-effective biomanufacturing of 1,2,4-butanetriol at the 1-L scale

Blake J. Rasor, Katherine A. Rhea, Isaiah D Richardson, John Tyler Lazar, Marilyn F. Slininger Lee, Ethan N. Walters, John R. Biondo, F. Joseph Kragl, David Garcia, Kyle Zolkin, John P. Davies, Matthew W. Lux, Ashty S. Karim, Michael C. Jewett

This paper develops a cell-free biosynthesis system for 1,2,4-butanetriol, achieving high productivity and yield at scale while reducing costs.

bioRxiv · Aug 4, 2026 · PreprintSynthetic Biology

A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection

Nin Rebecca Kang, Jisoo Im, John R. Biondo, Caitlin E. Sharpes, Katherine A. Rhea, Padric M. Garden, Juan J. Jaramillo Montezco, Alina Ringaci, Mark W. Grinstaff, Daniel A. Phillips, Aleksandr E. Miklos, Alexander A. Green

The paper introduces RAMONA, a rapid, modular nanobody assay for antigen detection that combines portability with high sensitivity and adaptability for diverse targets.

ACS Synthetic Biology · Sep 3, 2025Peer-reviewedBioengineering

Semi-automated Production of Cell-free Biosensors

Dylan M. Brown, Daniel A. Phillips, David C. Garcia, Aníbal Arce, Tyler J. Lucci, John P. Davies, Jacob T. Mangini, Katherine A. Rhea, Casey B. Bernhards, John R. Biondo, Steven M. Blum, Stephanie D. Cole, Jennifer A. Lee, Marilyn F. Slininger Lee, Nathan D. McDonald, , Julius B. Lucks

This paper develops a semi-automated method for manufacturing cell-free biosensors using liquid handling robotics, demonstrating comparable performance to manual methods.

ACS Synthetic Biology · Mar 12, 2025Peer-reviewedSynthetic Biology

Cell-Free Protein Expression in Polymer Materials

Marilyn F. Slininger Lee, Jennifer A. Lee, John R. Biondo, Jeffrey E. Lux, Rebecca M. Raig, Pierce N. Berger, Casey B. Bernhards, Danielle L. Kuhn, Maneesh K. Gupta, Matthew W. Lux

This paper demonstrates that dried cell-free protein synthesis reactions can withstand harsh conditions during polymer casting, enabling the creation of functional materials with spatially patterned genetic capabilities.

ACS Synthetic Biology · Mar 11, 2024Peer-reviewedSynthetic Biology