Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cells
F. V. Gorbenko, Irene Sala, Young-Yoon Lee, Lilly van de Venn, Charles D. Yeh, András Tálas, Tautvydas Karvelis, Gytis Druteika, Luca V. Bechter, Iryna Vykhlyantseva, Markus Schröder, Ana Gvozdenovic, Gerald Schwank, Virginijus Šikšnys, Jacob E. Corn
The paper improves the activity of compact RNA-guided nucleases in mammalian cells through directed evolution, resulting in variants with higher editing efficiency and reduced off-target effects.
Genome biology · Jun 16, 2026Peer-reviewedBioengineering