Virginijus Šikšnys

The paper improves the activity of compact RNA-guided nucleases in mammalian cells through directed evolution, resulting in variants with higher editing efficiency and reduced off-target effects.

This study reveals the structure of the type II-A CRISPR-Cas prespacer selection supercomplex and elucidates the role of Csn2 in Cas9-mediated spacer acquisition.

The paper shows that deoxydinucleotides activate the bacterial anti-phage defense system ApeA by binding to a conserved pocket, enabling broad protection against phage infection.

The paper shows short prokaryotic Argonautes, when associated with Sir2, deplete NAD+ to defend against invading DNA.

This study demonstrates that CRISPR endoribonucleases can efficiently knock down mRNA transcripts in zebrafish, with varying effectiveness across different EGFP-expressing lines.