BiochemistrybioRxiv
Unraveling the mechanisms of PAMless DNA interrogation by SpRY Cas9
G. N. Hibshman, J. P. Bravo, H. Zhang…·D. W. Taylor
This study investigates how SpRY-Cas9 recognizes DNA targets without requiring a PAM sequence, revealing structural and kinetic mechanisms that improve genome editing versatility.
DOI 10.1038/s41467-024-47830-3