Author

J. P. Bravo

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 9

Signature Assays, Organisms & Methods

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Approach:experimental assay· 9 Research Focus:host-pathogen & immune response· 4 Research Focus:protein structure prediction· 4 Assay:cryo-EM· 3 Research Focus:protein folding & dynamics· 3 Assay:structural biology· 3 Approach:microscopy & imaging analysis· 2 Organism:bacteria (other)· 1

Frequent Co-Authors

D. W. Taylor· 9 shared K. A. Johnson· 4 shared T. L. Dangerfield· 4 shared G. N. Hibshman· 2 shared A. Bardis· 1 shared A. Barendrecht· 1 shared A. Das· 1 shared A. M. Amieiro· 1 shared

Activity by Year

2020-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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9 shared papersBiochemistry

D. W. Taylor

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Author profile →

9 papers

Recent·Most cited

DNA targeting by compact Cas9d and its resurrected ancestor

R. Fregoso Ocampo, J. P. Bravo, T. L. Dangerfield, I. Noceda, S. Jirde, L. M. Alexander, A. Das, S. Nielsen, K. A. Johnson, C. T. Brown, C. N. Butterfield, D. S. Goltsman, D. W. Taylor

This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.

bioRxiv · Apr 8, 2024 · PreprintBiochemistry

Type III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages

J. Steens, J. P. Bravo, C. R. P. Salazar, C. Yildiz, A. M. Amieiro, S. Kostlbacher, S. H. P. Prinsen, C. Patinios, A. Bardis, A. Barendrecht, R. A. Scheltema, T. J. G. Ettema, J. van der Oost, D. W. Taylor, R. Staals

This study characterizes a type III-B CRISPR-Cas system in Haliangium ochraceum, revealing a proteolytic cascade involving SAVED-CHAT and PCaspase that activates immune responses against RNA targets.

bioRxiv · Jun 23, 2023 · PreprintBiochemistry

Unraveling the mechanisms of PAMless DNA interrogation by SpRY Cas9

G. N. Hibshman, J. P. Bravo, H. Zhang, T. L. Dangerfield, I. J. Finkelstein, K. A. Johnson, D. W. Taylor

This study investigates how SpRY-Cas9 recognizes DNA targets without requiring a PAM sequence, revealing structural and kinetic mechanisms that improve genome editing versatility.

bioRxiv · Jun 22, 2023Biochemistry

Modes of inhibition used by phage anti-CRISPRs to evade type I-C Cascade

R. E. O'Brien, J. P. Bravo, D. Ramos, G. N. Hibshman, J. T. Wright, D. W. Taylor

The paper presents a cryo-EM structure of the type I-C Cascade complex and describes how anti-CRISPR proteins inhibit it by blocking DNA binding.

bioRxiv · Jun 15, 2022 · PreprintBiochemistry

Assembly of multi-subunit fusion proteins into the RNA-targeting type III-D CRISPR-Cas effector complex

E. A. Schwartz, J. P. Bravo, L. A. Macias, C. L. McCafferty, T. L. Dangerfield, J. N. Walker, J. S. Brodbelt, P. C. Fineran, R. D. Fagerlund, D. W. Taylor

The paper determines the structure of a type III-D CRISPR-Cas effector complex and characterizes its RNA cleavage mechanism, revealing how multi-subunit fusion proteins assemble into an active RNA endonuclease.

bioRxiv · Jun 13, 2022 · PreprintBiochemistry

Large-scale structural rearrangements unleash indiscriminate nuclease activity of CRISPR-Cas12a2

J. P. Bravo, T. Hallmark, B. Naegle, C. L. Beisel, R. N. Jackson, D. W. Taylor

The paper reports structures of Cas12a2 to elucidate its activation pathway and mechanism of indiscriminate nuclease activity against various substrates.

bioRxiv · Jun 13, 2022 · PreprintBiochemistry

Structural basis for broad anti-phage immunity by DISARM

J. P. Bravo, C. A. Maldonado, F. L. Nobrega, S. J. Brouns, D. W. Taylor

This study determines the cryo-EM structures of the DISARM complex DrmAB and elucidates its mechanism of phage DNA recognition and activation.

bioRxiv · Dec 26, 2021Biochemistry

Structural basis for mismatch surveillance by CRISPR/Cas9

J. P. Bravo, M.-S. Liu, R. S. McCool, K. Jung, K. A. Johnson, D. W. Taylor

This study identifies a linear gRNA:DNA duplex conformation that prevents Cas9 activation in the presence of PAM-distal mismatches, offering insights for improving Cas9 specificity.

bioRxiv · Sep 14, 2021Biochemistry

Remdesivir is a delayed translocation inhibitor of SARS CoV-2 replication in vitro

J. P. Bravo, T. L. Dangerfield, D. W. Taylor, K. A. Johnson

This study shows remdesivir inhibits SARS-CoV-2 RNA polymerase by blocking RNA translocation after three base incorporations, leading to delayed chain termination.

bioRxiv · Dec 14, 2020Biochemistry