Molecular BiologyProceedings of the National Academy of SciencesPeer-reviewed
RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
Lin Di, Yusi Fu, Yue Sun…·Jianbin Wang
This paper introduces SHERRY, a new RNA-seq method that avoids second strand synthesis by using Tn5 transposase to directly fragment and prime RNA/DNA hybrids, improving reproducibility and GC uniformity.
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