RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Raymond W. Lee, Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, X. Sunney Xie, , Jianbin Wang
This paper introduces SHERRY, a new RNA-seq method that avoids second strand synthesis by using Tn5 transposase to directly fragment and prime RNA/DNA hybrids, improving reproducibility and GC uniformity.
Proceedings of the National Academy of Sciences · Jan 27, 2020Peer-reviewedMolecular Biology