Dong Wang

Structural Dynamics of RNA Polymerase II During Nucleotide Addition Cycle

This study presents 43 cryo-EM structures of RNAPII elongation complex during the nucleotide addition cycle, revealing structural dynamics and transition intermediates.

New Quantitative Cost-Impact Effectiveness Indexes to Assist in Publication Decisions by Researchers in the Open Access Era

The paper evaluates the cost-impact effectiveness of Open Access journals using three metrics to help researchers choose journals based on cost and impact.

RNA Polymerase II is a Polar Roadblock to a Progressing DNA Fork

This study investigates the polarity of RNA Polymerase II as a roadblock to DNA replication forks, showing that head-on collisions lead to stable binding and R-loop formation, with TFIIS facilitating hybrid removal.

Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions

This study uses cryo-EM to reveal a two-step mechanism of RNA polymerase I stalling at abasic DNA lesions, distinct from other lesion types.

Systematic Evaluation of Different R-loop Mapping Methods: Achieving Consensus, Resolving Discrepancies and Uncovering Distinct Types of RNA:DNA Hybrids

The paper evaluates eight R-loop mapping technologies, identifies biases and artifacts, and classifies R-loops into two distinct types based on RPA binding patterns.

RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids

This paper introduces SHERRY, a new RNA-seq method that avoids second strand synthesis by using Tn5 transposase to directly fragment and prime RNA/DNA hybrids, improving reproducibility and GC uniformity.

3.1A structure of yeast RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion solved using streptavidin affinity grids

The paper solves a high-resolution structure of a stalled RNA polymerase II elongation complex using streptavidin affinity grids to overcome air-water interface interactions.