Nanopore molecular trajectories of a eukaryotic reverse transcriptase reveal a long-range RNA structure sensing mechanism
A. Shaw, J. M. Craig, H. Amiri, J. Kim, H. E. Upton, S. C. Pimentel, J. R. Huang, S. Marqusee, J. H. Gundlach, K. Collins, C. J. Bustamante
The paper uses nanopore sequencing to study the single-nucleotide kinetics of a reverse transcriptase, revealing its ability to sense and destabilize RNA structures during cDNA synthesis.
bioRxiv · Apr 5, 2023 · PreprintBiophysics