Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter
R. Jia, C. Martens, M. Shekhar, S. Pant, G. A. Pellowe, A. M. Lau, H. E. Findlay, N. J. Harris, E. Tajkhorshid, P. J. Booth, A. Politis
The paper uses HDX-MS, mutagenesis, and MD simulations to show that substrate binding and protonation drive conformational changes in a transporter, while inhibitors prevent this transition.
bioRxiv · Apr 3, 2020Biochemistry