Author

N. J. Harris

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 7

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:cell signaling & trafficking· 5 Research Focus:protein folding & dynamics· 3 Assay:proteomics· 3 Approach:biophysical modeling· 2 Research Focus:drug response / drug discovery· 2 Approach:statistical model· 2 Research Focus:cancer & tumor microenvironment· 1

Frequent Co-Authors

J. E. Burke· 6 shared C. K. Yip· 4 shared M. A. Parson· 4 shared M. K. Rathinaswamy· 4 shared M. L. Jenkins· 4 shared K. D. Fleming· 3 shared U. Dalwadi· 3 shared H. Ranga-Prasad· 2 shared

Activity by Year

2020-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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6 shared papersBiochemistry

J. E. Burke

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Author profile →

7 papers

Recent·Most cited

Structure of Calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A

A. L. Shaw, S. Suresh, M. A. Parson, N. J. Harris, M. L. Jenkins, C. K. Yip, J. E. Burke

The paper reports a cryo-EM structure of Calcineurin bound to PI4KA, revealing direct interactions with both PI4KA and FAM126A and their conserved binding sequences.

bioRxiv · Apr 9, 2024Biochemistry

Allosteric activation or inhibition of PI3Kγ mediated through conformational changes in the p110γ helical domain

N. J. Harris, M. L. Jenkins, S.-E. Nam, M. K. Rathinaswamy, M. A. Parson, H. Ranga-Prasad, U. Dalwadi, B. E. Moeller, E. Sheeky, S. D. Hansen, C. K. Yip, J. E. Burke

This study identifies the helical domain of p110γ as an allosteric regulator of PI3Kγ activity, showing distinct roles in p110γ-p84 and p110γ-p101 complexes through phosphorylation or nanobody binding.

bioRxiv · Apr 12, 2023Biochemistry

Oncogenic mutations of PIK3CA lead to increased membrane recruitment driven by reorientation of the ABD, p85 and C-terminus

M. L. Jenkins, H. Ranga-Prasad, M. A. Parson, N. J. Harris, M. K. Rathinaswamy, J. E. Burke

This study identifies unique regulatory mechanisms by which oncogenic PIK3CA mutations enhance PI3K membrane recruitment and activation through structural reorientations.

bioRxiv · Apr 5, 2022 · PreprintBiochemistry

Biochemical insight into novel Rab-GEF activity of the mammalian TRAPPIII complex

N. J. Harris, M. L. Jenkins, U. Dalwadi, K. D. Fleming, S.-E. Nam, M. A. Parson, C. K. Yip, J. E. Burke

This study investigates the GEF activity of TRAPPIII and TRAPPII complexes toward Rab GTPases using biochemical and structural methods, revealing Rab specificity and membrane interaction mechanisms.

bioRxiv · Jun 1, 2021Biochemistry

HDX-MS optimized approach to characterize nanobodies as tools for biochemical and structural studies of class IB phosphoinositide 3-kinases

M. K. Rathinaswamy, K. D. Fleming, U. Dalwadi, E. Pardon, N. J. Harris, C. K. Yip, J. Steyaert, J. E. Burke

This paper uses HDX-MS to characterize nanobodies targeting PI3K{gamma} complexes, identifying epitopes and functional effects on lipid kinase activity and GPCR activation.

bioRxiv · Jun 1, 2021Biochemistry

Disease related mutations in PI3Kγ disrupt regulatory C-terminal dynamics and reveals a path to selective inhibitors

M. K. Rathinaswamy, Z. Gaieb, K. D. Fleming, C. Borsari, N. J. Harris, B. J. Moeller, M. P. Wymann, R. E. Amaro, J. E. Burke

This study investigates how mutations in PI3Kγ affect its regulatory dynamics and identifies a strategy for developing selective inhibitors.

bioRxiv · Dec 1, 2020Biochemistry

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

R. Jia, C. Martens, M. Shekhar, S. Pant, G. A. Pellowe, A. M. Lau, H. E. Findlay, N. J. Harris, E. Tajkhorshid, P. J. Booth, A. Politis

The paper uses HDX-MS, mutagenesis, and MD simulations to show that substrate binding and protonation drive conformational changes in a transporter, while inhibitors prevent this transition.

bioRxiv · Apr 3, 2020Biochemistry