Author

A. Politis

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biophysics· 5 Biochemistry· 4

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 7 Research Focus:protein folding & dynamics· 5 Assay:proteomics· 4 Approach:statistical model· 4 Organism:bacteria (other)· 3 Approach:deep learning· 3 Research Focus:cell signaling & trafficking· 2 Research Focus:protein structure prediction· 2

Frequent Co-Authors

E. Pyle· 3 shared Z. Ahdash· 3 shared A. M. Lau· 2 shared C. Martens· 2 shared I. Collinson· 2 shared K. Hansen· 2 shared P. J. Booth· 2 shared R. A. Corey· 2 shared

Activity by Year

2017-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersBiophysics

E. Pyle

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

Ligand-induced conformational changes in the β1-Adrenergic Receptor Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry

J. Toporowska, P. Kapoor, M. Musgaard, K. Gherbi, K. Sengmany, F. Qu, M. Soave, H.-Y. Yen, K. Hansen, A. Jazayeri, J. T. S. Hopper, A. Politis

This study uses HDX-MS to analyze structural dynamics of tβ1AR with different ligands, revealing ICL1 dynamics linked to GPCR activation and drug modality.

Nature Communications · Oct 18, 2024Peer-reviewedBiophysics

Integrative HDX-MS enables quantification of the conformational landscape of the sugar transporter XylE

R. Jia, R. Bradshaw, V. Calvaresi, A. Politis

This paper uses integrative HDX-MS and ensemble modeling to quantify conformational states of the sugar transporter XylE under different conditions.

bioRxiv · Jul 11, 2022 · PreprintBiophysics

Perturbed structural dynamics underlie inhibition and altered specificity of the multidrug efflux pump AcrB

E. Reading, Z. Ahdash, C. Fais, V. Ricci, X. Wang-Kan, E. Grimsey, J. W. Stone, G. Malloci, A. M. Lau, H. Findlay, A. Konijnenberg, P. J. Booth, P. Ruggerone, A. V. Vargiu, L. J. V. Piddock, A. Politis

The paper investigates the structural dynamics of the AcrB efflux pump, showing how inhibitors and mutations affect its function and substrate specificity.

bioRxiv · Apr 27, 2020Biochemistry

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

R. Jia, C. Martens, M. Shekhar, S. Pant, G. A. Pellowe, A. M. Lau, H. E. Findlay, N. J. Harris, E. Tajkhorshid, P. J. Booth, A. Politis

The paper uses HDX-MS, mutagenesis, and MD simulations to show that substrate binding and protonation drive conformational changes in a transporter, while inhibitors prevent this transition.

bioRxiv · Apr 3, 2020Biochemistry

HDX-MS reveals nucleotide-regulated, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

Z. Ahdash, E. Pyle, W. J. Allen, R. A. Corey, I. Collinson, A. Politis

This study uses HDX-MS to show that ATP binding causes SecY to open and SecA to close, while ATP hydrolysis reverses this, supporting a Brownian ratchet mechanism for protein translocation.

bioRxiv · Apr 1, 2019Biophysics

Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome

S. V. Faull, A. Lau, C. Martens, Z. Ahdash, H. Yebenes, C. Schmidt, F. Beuron, N. B. Cronin, E. P. Morris, A. Politis

The paper determines the structure and dynamics of CSN-CRL2 complexes using cryo-EM and mass spectrometry, revealing a stepwise activation mechanism.

bioRxiv · Nov 29, 2018Biochemistry

Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors

S. Pernigo, M. Chegkazi, Y. Yip, C. Treacy, G. Glorani, K. Hansen, A. Politis, M. P. Dodding, R. A. Steiner

This study identifies Y-acidic motifs recognized by KLC1TPR in kinesin-1, distinct from W-acidic motifs, and proposes a model for their differential activity promotion.

bioRxiv · May 14, 2018Biochemistry

Specific cardiolipin-SecY interactions are required for proton-motive-force stimulation of protein secretion

R. A. Corey, E. Pyle, W. J. Allen, M. Casiraghi, B. Miroux, I. Arechaga, A. Politis, I. Collinson

This study identifies and validates two conserved cardiolipin binding sites on the SecA-SecYEG complex, showing their role in ATP and PMF-driven protein secretion.

bioRxiv · Dec 6, 2017Biophysics

Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA

E. Pyle, A. C. Kalli, S. Amillis, Z. Hall, A. C. Hanyaloglu, G. Diallinas, B. Byrne, A. Politis

The paper investigates how membrane lipids affect the structure and function of the purine transporter UapA, showing that specific lipids stabilize its dimeric form and transport activity.

bioRxiv · Oct 20, 2017Biophysics