Sequence-dependent mechanochemical coupling of helicase translocation and unwinding at single-nucleotide resolution.
A. H. Laszlo, J. M. Craig, M. Gavrilov, R. Tippana, I. C. Nova, J. R. Huang, H. C. Kim, S. J. Abell, M. deCampos-Stairiker, J. W. Mount, J. L. Bowman, K. S. Baker, H. Higinbotham, D. Bobrovnikov, T. Ha, J. H. Gundlach
This study uses SPRNT to analyze how DNA sequence affects PcrA helicase kinetics during translocation and unwinding at single-nucleotide resolution.
bioRxiv · Feb 10, 2022Biophysics