Leveraging HILIC/ERLIC Separations for Online Nanoscale LC- MS/MS Analysis of Phosphopeptide Isoforms from RNA Polymerase II C-terminal Domain
Scott B. Ficarro, Deepash Kothiwal, Hyun Jin Bae, Isidoro Tavares, Gabriela Giordano, Stephen Buratowski, Jarrod A. Marto
This paper evaluates HILIC and ERLIC chromatography for resolving CTD phosphopeptide isoforms, finding ERLIC improves separation of phosphorylated peptides for LC-MS/MS analysis.
Journal of Chromatography B · Oct 8, 2024Peer-reviewedBiochemistry