Stephen Buratowski

The paper uses single-molecule fluorescence microscopy to show that TFIIH dissociates from RNA polymerase II before Spt4/5 binds, indicating Spt4/5 does not actively displace TFIIH.

This study uncouples the TFIIH kinase and core modules by splitting Tfb3, revealing that module coupling is necessary for localized CTD Serine 5 phosphorylation during transcription.

The paper uses single-molecule microscopy to show Mediator recruitment to promoters is synergistic when multiple activators bind, revealing a non-cooperative mechanism.

This study shows CDK12/13 inhibition disrupts nucleolar structure and causes aberrant IGS rRNA transcript expression due to impaired processing and degradation.

This paper evaluates HILIC and ERLIC chromatography for resolving CTD phosphopeptide isoforms, finding ERLIC improves separation of phosphorylated peptides for LC-MS/MS analysis.

This study uses single-molecule microscopy to analyze SAGA co-activator dynamics during transcription activation, revealing two distinct modes of interaction with DNA and activators.

The paper identifies Gds1 as an interacting partner of NuA4 that modulates H4 acetylation at ribosomal protein gene promoters in yeast.

The paper uses single-molecule microscopy to study RNA polymerase II pre-initiation complex assembly, revealing branched pathways and unexpected dynamics involving activators and transcription factors.

The paper shows that TAF subunits of TFIID bind downstream promoter DNA during transcription, promoting reinitiation independent of TBP or other factors.