Efficient single-copy HDR by 5’ modified long dsDNA donors
José Arturo Gutierrez-Triana, Tinatini Tavhelidse, Thomas Thumberger, Isabelle Thomas, Beate Wittbrodt, Tanja Kellner, Kerim Anlaş, Erika Tsingos, Joachim Wittbrodt
The paper demonstrates that modifying the 5' ends of long dsDNA donors enhances HDR efficiency in Medaka, enabling efficient single-copy integration through monomeric donor conformation.
eLife · Aug 20, 2018Peer-reviewedGenetics