Thomas Thumberger

The paper investigates how temperature cycles affect medaka embryonic development, revealing temperature-insensitive spatial patterns that compensate for temperature-sensitive temporal oscillations.

The paper investigates how morphogen gradients remain robust under temperature-induced changes in developmental tempo in medaka embryos, finding that reaction kinetics, not diffusion, drive this robustness.

The paper introduces faster variants of the Caprola tool for calcium-dependent fluorescent labeling, enabling more sensitive and efficient recording of neuronal activity in zebrafish.

This paper introduces a high-throughput optomotor response platform for analyzing vision in medaka and zebrafish, finding that albino medaka show heightened sensitivity to visual stimuli.

The paper establishes an auxin-inducible system to acutely knock down Pmm2 protein in medaka, mimicking hypomorphic mutations seen in PMM2-CDG patients.

The paper maps and validates key genetic loci influencing heart rate and function in fish, identifying potential targets for cardiac disease treatment.

The paper uses medaka fish to study genetic and environmental interactions in heart rate, identifying QTLs and validating genes, while showing limitations of GWAS in capturing complex genetic effects.

This study develops a high-throughput optomotor response assay in medaka larvae to quantify visual traits and detect strain-specific differences.

This paper introduces an approach called 'inception' that allows base editing at previously inaccessible sites by generating new PAM sequences through sequential combinatorial base editing.

This study demonstrates that 5-biotinylation of donor DNA enhances single-copy HDR in mice, achieving 19-26% efficiency in generating conditional alleles.

The paper develops a base editing framework in medaka and zebrafish to functionally validate SNVs, demonstrating its application in recreating cardiac phenotypes and validating candidate genes for congenital heart disease.

The paper introduces hei-tag, a tag that enhances CRISPR/Cas9 and base editor efficiency across multiple species and applications.

The paper introduces the Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel, a vertebrate genetic resource for genotype-to-phenotype studies, enabling controlled experiments on complex traits.

The paper analyzes genomic and epigenomic variation in the MIKK panel using long-read sequencing and pan-genome graphs, revealing novel structural variants and methylation patterns.

The paper identifies and validates 16 GWAS candidates with potential predictive power for human cardiovascular diseases using CRISPR/Cas9 in medaka.

The paper shows fish-derived organoids rapidly self-organize into retinal tissue, mimicking in vivo eye development, and highlights the impact of cell number and genetics on morphogenesis.

The paper models a hypo-N-glycosylation disorder in medaka fish, showing reduced N-glycan abundance and retinal degeneration, with mRNA rescue restoring viability.

The paper identifies genetic associations with myocardial trabecular complexity using UK Biobank data and shows a causal link to ventricular function and electrical activity.

The paper identifies Pcdh18a-positive tip cells that guide notochord formation in zebrafish through migration and signaling.

The paper introduces a low-cost DIY method for rapid nucleic acid extraction using cellulose filter discs in pipet tips, enabling fast and reliable genotyping of diverse samples.

The paper demonstrates that modifying the 5' ends of long dsDNA donors enhances HDR efficiency in Medaka, enabling efficient single-copy integration through monomeric donor conformation.

This study shows that maternal Wdr8 is essential for PCM assembly during embryonic mitosis in medaka, leading to developmental defects when disrupted.