Enhancing ML-based binder design with high-throughput screening: a comparison of mRNA and yeast display technologies
Zhiyuan Yao, McGuire Metts, Avery K. Huber, J Li, Tomoaki Kinjo, Henry Dieckhaus, Amrita Nallathambi, Albert A. Bowers, Brian A. Kuhlman
This study compares mRNA and yeast display technologies for evaluating ML-designed miniprotein binders, finding mRNA display better enriches slow dissociating binders and achieves higher library coverage.
bioRxiv · Feb 14, 2026 · PreprintBioengineering