Mutational and biophysical robustness in a pre-stabilized monobody
P. G. Chandler, L. L. Tan, B. T. Porebski, J. S. Green, B. T. Riley, S. S. Broendum, D. E. Hoke, R. J. Falconer, T. P. Munro, M. Buckle, C. J. Jackson, A. M. Buckle
This paper grafts binding loops from an Adnectin onto a pre-stabilized FN3Con scaffold to create a stable monobody that binds VEGFR2 with high affinity and long-term stability.
bioRxiv · Dec 14, 2020 · PreprintBiochemistry