Author

A. M. Buckle

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 6 Immunology· 3

Signature Assays, Organisms & Methods

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Organism:human· 5 Research Focus:protein folding & dynamics· 5 Assay:protein structure· 3 Approach:statistical model· 3 Assay:structural biology· 3 Research Focus:cell type annotation· 2 Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2

Frequent Co-Authors

B. T. Porebski· 5 shared B. T. Riley· 5 shared D. E. Hoke· 4 shared I. Kass· 4 shared J. Fodor· 4 shared N. A. Borg· 4 shared M. Buckle· 3 shared P. G. Chandler· 3 shared

Activity by Year

2018-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 38 mutual co-author links

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5 shared papersBiochemistry

B. T. Porebski

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Author profile →

9 papers

Recent·Most cited

Structure and dynamics of the autoantigen GAD65 in complex with the human autoimmune polyendocrine syndrome type 2-associated autoantibody b96.11

S. Stander, C. F. Reboul, S. N. Lee, D. Williams, P. G. Chandler, M. G. Costa, D. E. Hoke, J. D. Jimma, J. Fodor, G. Fenalti, S. I. Mannering, B. T. Porebski, P. Schofield, D. Christ, M. Buckle, , A. M. Buckle

This study characterizes the structure and dynamics of GAD65 and its interaction with autoantibody b96.11, revealing how intrinsic conformational flexibility drives autoantigenicity.

bioRxiv · Mar 18, 2024 · PreprintBiochemistry

Mutational and biophysical robustness in a pre-stabilized monobody

P. G. Chandler, L. L. Tan, B. T. Porebski, J. S. Green, B. T. Riley, S. S. Broendum, D. E. Hoke, R. J. Falconer, T. P. Munro, M. Buckle, C. J. Jackson, A. M. Buckle

This paper grafts binding loops from an Adnectin onto a pre-stabilized FN3Con scaffold to create a stable monobody that binds VEGFR2 with high affinity and long-term stability.

bioRxiv · Dec 14, 2020 · PreprintBiochemistry

Structural studies of thyroid peroxidase show the monomer interacting with autoantibodies in thyroid autoimmune disease

D. E. Williams, S. N. Le, D. E. Hoke, P. G. Chandler, M. Gora, M. Godlewska, J. P. Banga, A. M. Buckle

This study characterizes the structural and biophysical properties of thyroid peroxidase constructs and shows how autoantibodies bind the monomeric form, revealing conformational changes in the epitope.

bioRxiv · Dec 15, 2019Biochemistry

Phospho-tuning immunity through Denisovan, modern human, and mouse TNFAIP3 gene variants

N. W. Zammit, O. M. Siggs, P. Gray, K. Horikawa, S. R. Daley, D. B. Langley, D. Cultrone, E. K. Malle, S. N. Walters, J. E. Villanueva, J. Warren, A. Russell, M. J. Cowley, V. Gayevskiy, M. E. Dinger, , M. E. Craig

The paper identifies TNFAIP3 variants in humans, Denisovans, and mice that modulate A20 phosphorylation, revealing a genetic mechanism for balancing immunity and microbial tolerance.

bioRxiv · Mar 27, 2019 · PreprintImmunology

The Role of Conformational Dynamics in Abacavir-Induced Hypersensitivity Syndrome

J. Fodor, B. T. Riley, I. Kass, A. M. Buckle, N. A. Borg

This study uses molecular dynamics to show abacavir alters peptide conformations bound to HLA-B*57:01, influencing immune self-reactivity and hypersensitivity.

bioRxiv · Feb 27, 2019Immunology

A metal-dependent switch moderates activity of the hexameric M17 aminopeptidases.

N. Drinkwater, W. Yang, B. T. Riley, B. K. Hayes, K. Kannan Sivaraman, T. R. Malcolm, S. C. Atkinson, N. A. Borg, I. Kass, A. M. Buckle, S. McGowan

This study identifies a metal-dependent regulatory mechanism in PfA-M17 aminopeptidase, showing how oligomerization and metal center re-modelling control proteolytic activity.

bioRxiv · Feb 21, 2019 · PreprintBiochemistry

Reactive Centre Loop Dynamics and Serpin Specificity

E. M. Marijanovic, J. Fodor, B. T. Riley, B. T. Porebski, M. G. Costa, I. Kass, D. E. Hoke, S. McGowan, A. M. Buckle

This study investigates serpin specificity by engineering the reactive centre loop (RCL) of a synthetic serpin, finding that RCL sequence alone does not determine specificity, and that local electrostatics and dynamics also play critical roles.

bioRxiv · Aug 14, 2018Biochemistry

Structural Capacitance in Protein Evolution and Human Diseases

C. Li, L. V. Clark, R. Zhang, B. T. Porebski, J. M. McCoey, N. A. Borg, G. I. Webb, I. Kass, M. Buckle, J. Song, A. Woolfson, A. M. Buckle

The paper identifies structural capacitance as a new mechanism in protein evolution that generates microstructure in disordered regions, linking it to disease and evolutionary adaptability.

bioRxiv · Feb 22, 2018Biochemistry

Previously Hidden Dynamics at the TCR-pMHC Interface Revealed

J. Fodor, B. T. Riley, N. A. Borg, A. M. Buckle

The paper reveals hidden conformational dynamics in TCR-pMHC interactions using ensemble refinement of X-ray crystallographic data, enhancing functional interpretations of immune responses.

bioRxiv · Feb 21, 2018Immunology