J. V. Rodrigues

Switching an active site helix in dihydrofolate reductase reveals limits to sub-domain modularity

This study replaces a helical segment in DHFR with sequences from unrelated proteins, showing that while chimeras fold, their activity and stability are reduced, highlighting limits to sub-domain modularity.

Development of antibacterial compounds that block evolutionary pathways to resistance

This study identifies a compound that inhibits both wild-type and TMP-resistant variants of E. coli DHFR, delaying resistance development through efflux pump duplication.

Adaptation to mutational inactivation of an essential E. coli gene converges to an accessible suboptimal fitness peak.

The paper shows that adaptation to mutational inactivation of an essential E. coli gene converges to a suboptimal fitness peak through specific metabolic and proteomic changes.

Comparison Between Normobaric Hypoxia Altitude Simulation Test and Altitude Hypoxia Predictive Equations in Cystic Fibrosis Patients

The paper compares normobaric hypoxia altitude simulation test results with five predictive equations in cystic fibrosis patients to assess their accuracy in predicting in-flight hypoxia.