Handling difficult cryo-ET samples: A study with primary neurons from Drosophila melanogaster
J. Y. Kim, J. E. Yang, J. W. Mitchell, L. A. English, S. Z. Yang, T. Tenpas, E. W. Dent, J. Wildonger, E. R. Wright
This study addresses challenges in preparing Drosophila melanogaster neurons for cryo-ET by optimizing substrate selection, micropatterning, data collection, and fixation.
bioRxiv · Jul 10, 2023 · PreprintBiophysics