Author

James E. DiCarlo

Primary ORCID profile (0000-0003-4728-3186 · 1 paper)Unclaimed ORCID profile · follow to get notified of new preprints

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Same name, identified by ORCID

James E. DiCarloiD1 paper · Brigham and Women's Hospital

Biology Disciplines

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Bioengineering· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Organism:E. coli· 1 Approach:experimental assay· 1 Approach:genetic perturbation / CRISPR· 1 Research Focus:genome assembly· 1

Frequent Co-Authors

George M. Church· 2 shared Alejandro ChaveziD· 1 shared Alexandra GonzalesiD· 1 shared Angela Tung· 1 shared Barry L. Wanner· 1 shared Cameron L. GardneriD· 1 shared Christian Kaas· 1 shared Christopher J. Gregg· 1 shared

Activity by Year

2016-2017 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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2 shared papersBioengineering

George M. Church

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2 papers

Recent·Most cited

High-throughput creation and functional profiling of eukaryotic DNA sequence variant libraries using CRISPR/Cas9

Xiaoge Guo, Alejandro Chavez, Angela Tung, Yingleong Chan, Ryan Cecchi, Santiago Lopez Garnier, Christian Kaas, Eric Kelsic, Max Schubert, James E. DiCarlo, James J. Collins, George M. Church

The paper introduces a Cas9-based method to create and functionally profile eukaryotic DNA variant libraries, enabling efficient analysis of genetic alterations.

bioRxiv · Sep 29, 2017 · PreprintBioengineering

Assembly of Radically Recoded E. coli Genome Segments

Julie E. Norville, Cameron L. Gardner, Eduardo Aponte, Conor K. Camplisson, Alexandra Gonzales, David K. Barclay, Katerina A. Turner, Victoria Longe, Maria Mincheva, Jun Teramoto, Kento Tominaga, Ryota Sugimoto, James E. DiCarlo, Marc Guell, Eriona Hysolli, , George M. Church

This paper introduces CAGE 2.0, a platform for scarless assembly of recoded E. coli genome segments, enabling efficient and safe genome engineering.

bioRxiv · Aug 31, 2016 · PreprintSynthetic Biology