Marker-free coselection for successive rounds of prime editing in human cells
Sébastien Levesque, Diana Mayorga, Jean-Philippe Fiset, Claudia Goupil, Alexis Duringer, A Loiselle, Eva Bouchard, Daniel Agudelo, Yannick Doyon
This paper introduces a coselection strategy for prime editing in human cells using the Na+/K+ ATPase to enable efficient multiallelic editing and reduce the need for pegRNA optimization.
Nature Communications · Oct 7, 2022Peer-reviewedMolecular Biology