Sébastien Levesque

This study shows that disrupting autophagy in CAR-T cells enhances their metabolic activity and improves their ability to reject ovarian tumors in vivo.

This paper introduces a method for targeted genomic integration and rearrangement in human cells using CRISPR-targeted dual flap synthesis, enabling DNA sequence assembly without requiring double-stranded DNA donors or cell cycle progression.

This study shows that gene editing of HSCs without ex vivo culture reduces genotoxicity while maintaining therapeutic efficacy for beta-hemoglobinopathy treatment.

This study shows that nucleotide metabolism limits prime editing efficiency in HSPCs and that deoxynucleoside supplementation and Vpx-mediated SAMHD1 degradation enhance it.

This paper introduces a coselection strategy for prime editing in human cells using the Na+/K+ ATPase to enable efficient multiallelic editing and reduce the need for pegRNA optimization.

This paper engineers CRISPR-Cas9 variants from Streptococcus thermophilus to target diverse A-rich PAMs, enabling efficient genome editing in mice.