Jennifer N. Hennigan

This paper describes a systems engineering approach to improve the scalable and robust production of nanobodies in E. coli through dynamic control of reduction potential and two-stage purification.

This paper demonstrates that dynamic deregulation of central metabolism in E. coli improves process robustness and scalability for industrial chemical production.

This paper describes a method to improve protein expression and purification in E. coli by inducing autolysis and DNA/RNA hydrolysis via phage lysozyme and endonuclease, achieving >90% protein release.

The paper tests the robustness of PhoB-regulated promoters in E. coli across different media and identifies promoters with consistent expression for two-stage autoinduction.