Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms
Matthew J. Szarzanowicz, Michael Busche, Ziyu Dai, Nathan Lapp, Gina M. Geiselman, Jiayuan Jia, Lucas M. Waldburger, Yan Liang, Guoliang Yuan, Rylan D. Duong, Kyle R. Pomraning, Nathan J. Hillson, Jacob O. Brunkard, Blake Alexander Simmons, John M. Gladden, , Patrick M. Shih
This study shows that binary vector origin of replication family, not copy number, determines Agrobacterium-mediated transformation outcomes across plants and fungi.
bioRxiv · Nov 18, 2025 · PreprintPlant Biology