Author

Guoliang Yuan

Pacific Northwest National LaboratoryORCID 0000-0002-6562-8769Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Microbiology· 1 Plant Biology· 1

Signature Assays, Organisms & Methods

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Research Focus:clustering· 2 Approach:experimental assay· 2 Organism:fungi (other)· 2 Research Focus:cell type annotation· 1 Research Focus:development & morphogenesis· 1 Research Focus:gene regulatory network inference· 1 Research Focus:genome assembly· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

Joonhoon KimiD· 2 shared Beth A. Hofstad· 1 shared Blake Alexander SimmonsiD· 1 shared Gina M. GeiselmaniD· 1 shared Jacob O. BrunkardiD· 1 shared Jeffrey J. CzajkaiD· 1 shared Jiayuan Jia· 1 shared John M. GladdeniD· 1 shared

Activity by Year

2024-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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2 shared papersPlant Biology

Joonhoon KimORCID

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2 papers

Recent·Most cited

Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms

Matthew J. Szarzanowicz, Michael Busche, Ziyu Dai, Nathan Lapp, Gina M. Geiselman, Jiayuan Jia, Lucas M. Waldburger, Yan Liang, Guoliang Yuan, Rylan D. Duong, Kyle R. Pomraning, Nathan J. Hillson, Jacob O. Brunkard, Blake Alexander Simmons, John M. Gladden, , Patrick M. Shih

This study shows that binary vector origin of replication family, not copy number, determines Agrobacterium-mediated transformation outcomes across plants and fungi.

bioRxiv · Nov 18, 2025 · PreprintPlant Biology

CRISPR-Cas9/Cas12a Systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger

Guoliang Yuan, Shuang Hui Deng, Jeffrey J. Czajka, Ziyu Dai, Beth A. Hofstad, Joonhoon Kim, Kyle R. Pomraning

This paper compares CRISPR-Cas9 and Cas12a systems for genome editing and large fragment deletions in Aspergillus niger, showing Cas12a's higher efficiency with single gRNA and both systems achieving 100% efficiency with two gRNAs.

Frontiers in Bioengineering and Biotechnology · Oct 16, 2024Peer-reviewedMicrobiology