Lucas M. Waldburger

Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells

This paper uses synthetic biology to engineer BCG to produce HMBPP, enhancing Vγ9Vδ2 T cell expansion without feedback inhibition, offering a strategy to improve TB vaccine efficacy.

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression

The paper models variability in Nicotiana benthamiana transient expression and provides guidelines for minimizing it through experimental design and power analysis.

Multiplexed profiling of transcriptional regulators in plant cells

This study introduces ENTRAP-seq, a method to measure transcriptional regulator activity in plants, and identifies novel regulatory domains in viral proteins.

Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms

This study shows that binary vector origin of replication family, not copy number, determines Agrobacterium-mediated transformation outcomes across plants and fungi.

Diversity-driven biochemical survey reveals dimeric structural origin of rubisco

The paper characterizes the structural and functional diversity of rubisco by synthesizing and analyzing diverse sequences, revealing a dimeric origin and a novel large rubisco structure.

Evolutionary discovery and characterization of fungal transcriptional activators using active learning

The paper develops ADhunter, a model to identify and quantify transcriptional activators in fungal genomes, expanding functional annotations across non-model species.

Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana

The pGinger family of expression plasmids

The paper introduces a family of 43 expression plasmids for precise gene expression in gram-negative bacteria, with constitutive and inducible systems tested in Escherichia coli and Pseudomonas putida.