CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Neil C. Dalvie, Timothy R. Lorgeree, Yuchen Yang, Sergio A. Rodriguez‐Aponte, Charles A. Whittaker, Joshua A. Hinckley, John J. Clark, Amanda M. Del Rosario, Kerry Routenberg Love, John Christopher Love
The paper uses CRISPR-Cas9 to knock out endogenous secreted proteins in Komagataella phaffii, increasing recombinant protein production by 20x.
Microbial Cell Factories · Jul 31, 2024Peer-reviewedBioengineering