Organelle-selective click labeling coupled with flow cytometry allows high-throughput CRISPR screening of genes involved in phosphatidylcholine metabolism
M. Tsuchiya, N. Tachibana, K. Nagao, T. Tamura, I. Hamachi
This paper introduces O-ClickFC, a method combining organelle-selective click labeling and flow cytometry for CRISPR screening of genes involved in phosphatidylcholine metabolism, identifying both known and novel genes.
bioRxiv · Apr 18, 2022 · PreprintCell Biology