Nanoscale 3D DNA tracing in single human cells visualizes loop extrusion directly in situ
K. S. Beckwith, O. Oedegaard Fougner, N. R. Morero, C. Barton, F. Schueder, W. Tang, S. Alexander, J.-M. Peters, R. Jungmann, E. Birney, J. Ellenberg
This study uses nanoscale 3D imaging to show that DNA folds as flexible coils without loop extruding enzymes, but forms reproducible loops with Cohesin and CTCF.
bioRxiv · Apr 12, 2021 · PreprintCell Biology